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Image Search Results
Journal: Nature Communications
Article Title: Spatial transcriptomics deconvolution at single-cell resolution using Redeconve
doi: 10.1038/s41467-023-43600-9
Figure Lengend Snippet: a overview of Redeconve workflow for deconvoluting spatial transcriptomics data. Redeconve requires sc/snRNA-seq data together with spatial transcriptomics data as input and performs deconvolution by solving a regularized non-negative least regression model with the aims to estimate cellular composition across spots at single-cell resolution. b heatmap illustrated median spot-level Spearman’s correlation of cell type proportions among different algorithms on a human breast cancer dataset. c Sankey diagram demonstrated the cell-type and single-cell resolutions of Redeconve results on human breast cancer and mouse cerebellum datasets, respectively. The bar height of cell types or single cells refer to their estimated abundance after deconvolution. d line chart of cosine similarities between observed and reconstructed expression profiles per spot based on six ST datasets. N = 4039, 2426, 428, 36550, 2987 and 39431 spots for human lymph nodes, human breast cancer, PDAC (pancreatic ductal adenocarcinoma), human testis, mouse brain and mouse cerebellum respaectively. Spots were sorted by an ascending order of the cosine similarities. e Pearson correlation of cell abundances between Redeconve and the cell counts per spot based on a mouse brain dataset. The ground truth cell counts per spot was obtained by nucleus counting of cell segmentation image . f computational efficiency of different deconvolution-based and mapping-based algorithms on a human lymph nodes dataset. Source data of 1c-e are provided as a file.
Article Snippet: The
Techniques: Expressing